Journal:

Article Title: Global Gene Expression Profiling of End-Stage Dilated Cardiomyopathy Using a Human Cardiovascular-Based cDNA Microarray

doi:

Figure Lengend Snippet: Real-time RT-PCR results of selected genes identified by the microarray analysis. a: Fold difference expression of each gene in DCM heart samples relative to nonfailing (NF) samples. ANP, atrial natriuretic peptide (positive control); CNN3870 and HA6788, novel heart transcripts; EIF1AY, elongation initiation factor 1A, Y isoform; CaCKK, Ca2+/calmodulin kinase kinase; InosK, inositol 1,4,5-triphosphate-3-kinase; InosTR, inositol 1,4,5-triphosphate receptor; CCaATPase, cardiac Ca2+ ATPase. b: Mean relative mRNA population of each gene from a versus GAPDH in DCM (n = 7) and NF (n = 5) samples. †, P < 0.01; *, P < 0.05 versus NF.

Article Snippet: 8 To construct the CardioChip, we took 113 of these microplates containing 10,848 nonredundant PCR products (Table 1) , spotted them onto CMT-GAPS-II amino-silane-coated glass microarray slides (Corning) using the GMS 417 arrayer (Affymetrix, Santa Clara, CA) and postprocessed the arrays.

Techniques: Quantitative RT-PCR, Microarray, Expressing, Positive Control

Journal: BMC Systems Biology

Article Title: A computational framework for gene regulatory network inference that combines multiple methods and datasets

doi: 10.1186/1752-0509-5-52

Figure Lengend Snippet: Inference of of biologically relevant networks . Gene regulatory networks obtained from the glioblastoma (panels A, B) and E. coli acid stress datasets (panels C and D). Networks obtained from SOO (panels A and C) and MOO (panels B and D) procedures are shown.

Article Snippet: Labelled probes (80 pmol) were then hybridized on Operon E. coli Ultra GAPS microarray slides (Corning, USA).

Techniques:

Journal: PLoS ONE

Article Title: Genome-Wide Transcriptional Response of the Archaeon Thermococcus gammatolerans to Cadmium

doi: 10.1371/journal.pone.0041935

Figure Lengend Snippet: Growth of T. gammatolerans EJ3 T in artificial seawater medium (ASW-AA-Cyst) in the absence of Cd (•), or after addition of 50 µM (♦), 100 µM (▴), 500 µM (-) and 1 mM (▪) Cd. Blue dotted lines indicate the timepoints (30, 120, 270 min) for microarrays analyses. The values are the mean of three independent cultures (standard deviation (SD) ≤10%).

Article Snippet: After a denaturation step for 2 min at 95°C, the concentrated Cy3 and Cy5 cDNA were dropped onto glass slide microarrays and incubated in a hybridization chamber (Corning) for 17 h at 50°C.

Techniques: Standard Deviation